Self-contained and modular structured illumination microscope
نویسندگان
چکیده
منابع مشابه
Flexible structured illumination microscope with a programmable illumination array.
Structured illumination microscopy (SIM) has grown into a family of methods which achieve optical sectioning, resolution beyond the Abbe limit, or a combination of both effects in optical microscopy. SIM techniques rely on illumination of a sample with patterns of light which must be shifted between each acquired image. The patterns are typically created with physical gratings or masks, and the...
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The design of an Adaptive Optics (AO) Structured Illumination (SI) microscope is presented. Two key technologies are combined to provide effective super-resolution at significant depths in tissue. AO is used to measure and compensate for optical aberrations in both the system and the tissue by measuring the optical path differences in the wavefront. Uncorrected, these aberrations significantly ...
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This paper explores a new application of structured illumination to determine the position of a microscope stage. Measuring stage position accurately is important for any experiment that spans multiple fields of view. The structured illumination pattern is provided by a design printed on the microscope slide or cover slip that modulates the image of the specimen. A novel application of frequenc...
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We propose a super resolution imaging technique called plasmonic structured illumination microscopy (PSIM), which combines the structured illumination microscopy technique with the tunable surface plasmon interference. Because of the high-resolution enabled by using surface plasmon interference as an illumination source, PSIM possesses higher image resolving power compared with conventional str...
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Two types of laser illumination in live cell microscopy with a focus on the sample or in the aperture plane of the microscope objective lens are distinguished. For the second case two examples are described, namely light scattering microscopy with angular resolution and Structured Illumination Microscopy (SIM) with two interfering laser beams. Appropriate applications include morphological stud...
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ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2021
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.423492